DNA

Part:BBa_K2796046

Designed by: Shuangshuang Pu   Group: iGEM18_LZU-CHINA   (2018-10-04)


ECOB-miniCMV- hsa-miR-135b-3p

By adding biotin, ECOB-miniCMV promoter can be activated and express the downstream interest gene hsa-miR-135b-3p.Then it will be cut by dicer enzyme and become mature microRNA, thus playing the role of anti-tumor acitivity.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 222
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 202
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 256


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Parameters
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